TB-500 2 mg Vial: Reconstitution and Dosing Chart

How much bacteriostatic water to add to a 2 mg vial of TB-500, the concentration you get, and a full dose-to-units chart.

Medical review pendingSynthetic fragment of thymosin beta-4Updated 21 Oct 2026

Vial size: 2 mg
With 2 mL water: 1 mg/mL; common choice
10 units contain: 0.1 mg; at 2 mL, U-100
Whole vial: 200 units; 2 mL on a U-100 scale

How much water to add

Bacteriostatic water only changes the concentration, not the amount of TB-500 in the vial. More water means more units per dose (easier to read), less water means fewer.

TB-500 2 mg vial: concentration by water added
Water added	Concentration	10 units contain	1 mg is	Whole vial
1 mL	2 mg/mL	0.2 mg	50 u	100 u
2 mL	1 mg/mL	0.1 mg	100 u	200 u
2.5 mL	0.8 mg/mL	0.08 mg	125 u	250 u
3 mL	0.667 mg/mL	0.067 mg	150 u	300 u

Calculator and full dosing table

Preset to the 2 mg vial. Change any value; the table below the result updates with it.

1Vial size

2Bacteriostatic water added

3Dose per injection

4Syringe (U-100)

Draw to

200units

= 2 mL on a 1 mL U-100 insulin syringe

This dose is more than the syringe holds. Choose a larger syringe or add less water.

2 mg vial2 mL water2 mg dose

Concentration: 1 mg/mL
Doses per vial: 1
Weeks (1 dose/wk): 1

Math only. It does not recommend a dose.

Dosing table for a 2 mg vial

Syringe units (U-100) for each dose, by how much bacteriostatic water you add.

TB-500: U-100 syringe units per dose for a 2 mg vial
Dose	1 mL water	2 mL water	2.5 mL water	3 mL water
0.5 mg	25	50	62.5	75
1 mg	50	100	125	150
2 mg	100	200	250	300

How the math works

Concentration is the vial's milligrams divided by the water you add. Volume to draw is your dose divided by that concentration. On a U-100 syringe, 1 mL is 100 units, so units = mL × 100.

2 mg ÷ 2 mL = 1 mg/mL
2 mg ÷ 1 mg/mL = 2 mL
2 mL × 100 = 200 units

200 units · 2 mL · 2 mg from 2 mg + 2 mL

Math only. The amounts in these tables are reference values for the arithmetic, not dose recommendations.

Doses to set a 2 mg vial against

A horse doping-control study gives a single product dose containing 10 mg of N-acetylated LKKTETQ, the only absolute TB-500 amount in the cited papers. It is an animal amount and is not converted to a human one.^[3]

Human intravenous dosing in the cited trials was of full-length thymosin beta-4 by weight, not TB-500 by vial.^[4]

What 2 mg gives

Mixed with 1, 2 or 3 mL of water, a 2 mg TB-500 vial is 2, 1, 0.667 mg/mL, so one U-100 unit holds 20, 10, 6.67 mcg.

Calculated from vial mg 2; water mL 1, 2, 3; units per mL 100. Formula: mg/mL = vialMg / waterMl; mcg per unit = mg/mL x 1000 / 100

Is 2 mg a labelled strength?

No regulator lists a thymosin beta product, so there is no labelled strength to compare against.^[5]

With 0 matching applications in Drugs@FDA for thymosin beta, a 2 mg vial cannot match a labelled strength.

Calculated from vial mg 2; drugs fda matches 0. Formula: labelled strengths = 0

Common questions

How much water goes in a 2 mg vial of TB-500?

That choice only changes the concentration. With 2 mL, a 2 mg vial is 1 mg/mL; with 1 mL it is 2 mg/mL. Less water means fewer units per dose and harder-to-read marks for small doses.

How many units is 1 mg from a 2 mg vial?

With 2 mL of water, 1 mg is 100 units on a U-100 syringe. With other water amounts, see the table.

How many doses are in a 2 mg vial?

Divide the vial size by the dose (in the same unit). The calculator on this page shows the number of whole doses for any dose you pick.

How much bacteriostatic water for a 2 mg of TB-500 vial?

Water volume only sets concentration: 1, 2 or 3 mL gives 2, 1, 0.667 mg/mL for the 2 mg vial.

Calculated from vial mg 2; water mL 1, 2, 3. Formula: mg/mL = vialMg / waterMl

More on TB-500

DosageDoses used in published studies, with the source for each.CalculatorVial, water and dose to syringe units.Side effectsWhat labels and studies report, and what is unknown.BenefitsClaimed benefits next to what published studies measured.How it worksTarget, mechanism and how long it acts, from the label or studies.TopicalStudies that gave TB-500 as creams and serums, and what is approved.

Sources

1Metric (SI) Prefixes (NIST Reference on Constants, Units, and Uncertainty)National Institute of Standards and Technology (NIST) · 2025physics.nist.govAccessed 2 Oct 2026
2BASAGLAR (insulin glargine) injection, prescribing information (DailyMed)U.S. Food and Drug Administration / DailyMed (Eli Lilly and Company label) · 2026dailymed.nlm.nih.govAccessed 2 Oct 2026
3Doping control analysis of TB-500, a synthetic version of an active region of thymosin β₄, in equine urine and plasma by liquid chromatography-mass spectrometry.Journal of chromatography. A · 2012PMID 23084823Accessed 2 Oct 2026
4A first-in-human, randomized, double-blind, single- and multiple-dose, phase I study of recombinant human thymosin β4 in healthy Chinese volunteers.Journal of cellular and molecular medicine · 2021PMID 34346165Accessed 2 Oct 2026
5Drugs@FDA (openFDA) search: active ingredient "thymosin beta"U.S. Food and Drug Administration · 2026api.fda.govAccessed 2 Oct 2026

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