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Peptide calculator

Enter the vial size, the bacteriostatic water you add and your dose. The calculator shows how many units to draw on an insulin syringe, the volume in mL and how many doses the vial holds.

Medically reviewed by Juan Pablo Frías, MD · · DisclosureUpdated Educational use only
Dose unit
Syringe scale
1Vial size
mg
2Bacteriostatic water added
mL
3Dose per injection
mg
4Syringe (U-100)
Draw to
50units
= 0.5 mL on a 1 mL U-100 insulin syringe
10 mg vial2 mL water2.5 mg dose
Concentration
5 mg/mL
Doses per vial
4
Weeks (1 dose/wk)
4
Math only. It does not recommend a dose.

50 units (0.5 mL) on a 1 mL U-100 syringe: 2.5 mg from a 10 mg vial + 2 mL water.

Dosing table for a 10 mg vial

Syringe units (U-100) for each dose, by how much bacteriostatic water you add.

U-100 syringe units per dose for a 10 mg vial
Dose1 mL water2 mL water2.5 mL water3 mL water
0.25 mg2.556.257.5
0.5 mg51012.515
1 mg10202530
2 mg20405060
2.5 mg255062.575
5 mg50100125150
7.5 mg75150187.5225
10 mg100200250300

How the math works

Concentration is the vial's milligrams divided by the water you add. Volume to draw is your dose divided by that concentration. On a U-100 syringe, 1 mL is 100 units, so units = mL × 100.

10 mg ÷ 2 mL = 5 mg/mL2.5 mg ÷ 5 mg/mL = 0.5 mL0.5 mL × 100 = 50 units
50 units · 0.5 mL · 2.5 mg from 10 mg + 2 mL
By peptide

Calculators for each peptide

Vial sizes are preset for each peptide, and the dose unit (mg or mcg) matches how the peptide is usually discussed.

GLP-1 & metabolic

Tirzepatide

GLP-1 / GIP dual agonist
FDA approved (Mounjaro, Zepbound)
Vials: 5, 10, 15, 20, 30, 40, 50, 60, 120 mg · dose in mg

Retatrutide

GLP-1 / GIP / glucagon triple agonist
Investigational · phase 3
Vials: 5, 10, 12, 15, 20, 24, 30, 36, 40, 50, 60 mg · dose in mg

MOTS-c

Mitochondrial-derived peptide
PCAC voted 7–5 for 503A (Jul 2026)
Vials: 5, 10, 20, 40 mg · dose in mg

Healing & recovery

TB-500

Synthetic fragment of thymosin beta-4
PCAC voted 8–6 for 503A (Jul 2026)
Vials: 2, 5, 10, 20 mg · dose in mg

BPC-157

Gastric pentadecapeptide
PCAC voted 8–6 for 503A (Jul 2026)
Vials: 2, 5, 10, 20 mg · dose in mcg

KPV

Anti-inflammatory tripeptide
PCAC voted 8–6 for 503A (Jul 2026)
Vials: 5, 10, 20 mg · dose in mcg

Growth hormone

Cognitive & sleep

Semax

ACTH(4-10) analog
PCAC voted 8–5 for 503A (Jul 2026)
Vials: 5, 10, 30 mg · dose in mcg

Skin & hair

GHK-Cu

Copper tripeptide
Injectable off Category 2 (Apr 2026)
Vials: 50, 100, 200 mg · dose in mg

Longevity

SS-31

Mitochondria-targeting peptide
FDA accelerated approval for Barth syndrome (Sep 2025)
Vials: 10, 25, 50 mg · dose in mg

Sexual & hormonal health

Other

Method

How to use the calculator

A worked example: a 10 mg vial, 2 mL of bacteriostatic water and a 2.5 mg dose on a 1 mL U-100 syringe.

Reconstitution guide
01

Read the vial

The label gives the total amount in mg, here 10 mg. Reconstituting does not change it.

02

Find the concentration

Divide the mg by the water you add: 10 mg ÷ 2 mL = 5 mg/mL.

03

Find the volume

Divide the dose by the concentration: 2.5 mg ÷ 5 mg/mL = 0.50 mL.

04

Convert to units

On a U-100 syringe 1 mL is 100 units: 0.50 mL × 100 = 50 units. The vial holds 4 such doses.

Syringes

How to read an insulin syringe

Insulin syringes are marked in units, not mL. The scale printed on the barrel tells you how many units make 1 mL.

Insulin syringe guide

0.3 mL U-100 syringe

Holds 30 units. Each unit is 0.01 mL, so 10 units is 0.1 mL.

Filled to 10 units (33.33% of the barrel).

0.5 mL U-100 syringe

Holds 50 units. Each unit is 0.01 mL, so 10 units is 0.1 mL.

Filled to 10 units (20% of the barrel).

1 mL U-100 syringe

Holds 100 units. Each unit is 0.01 mL, so 10 units is 0.1 mL.

Filled to 10 units (10% of the barrel).

U-40 syringes

A 1 mL U-40 syringe holds 40 units, so each unit is 0.025 mL and 10 units is 0.25 mL. A units number from a U-100 chart drawn on a U-40 syringe gives 2.5 times the volume.

Switch the calculator to U-40 above to get U-40 units.
Converters

Quick conversions

All converters

Common questions

How many units is 2.5 mg?

It depends on the concentration. In a 10 mg vial mixed with 2 mL of water (5 mg/mL), 2.5 mg is 50 units (0.50 mL) on a U-100 syringe. With 1 mL of water it is 25 units; with 3 mL it is 75 units.

How much bacteriostatic water do I add to a 10 mg vial?

Any volume works mathematically; the water only sets the concentration. 1 mL gives 10 mg/mL, so 1 mg is 10 units; 2 mL gives 5 mg/mL, so 1 mg is 20 units; 3 mL gives 3.33 mg/mL, so 1 mg is 30 units. More water makes small doses easier to measure but means more volume per injection.

How many units is 250 mcg?

250 mcg is 0.25 mg. From a 5 mg vial with 2 mL of water it is 10 units (0.10 mL) on a U-100 syringe; from a 10 mg vial with 2 mL it is 5 units.

How many mL is 10 units on an insulin syringe?

On a U-100 syringe 10 units is 0.1 mL. On a U-40 syringe the same 10 units is 0.25 mL.

What is the difference between U-100 and U-40 syringes?

A U-100 syringe has 100 unit marks per mL; a U-40 syringe has 40. The same 0.25 mL reads 25 units on U-100 and 10 units on U-40. Reading a units number on the wrong scale changes the volume by 2.5 times, so always use the scale printed on your syringe.

How many doses are in a vial?

Divide the vial's milligrams by the dose: 10 mg ÷ 2.5 mg = 4 doses. The water you add does not change the number of doses, only the volume of each one.

Sources

  1. 1Metric (SI) Prefixes (NIST Reference on Constants, Units, and Uncertainty)National Institute of Standards and Technology (NIST) · 2025physics.nist.govAccessed 2 Oct 2026
  2. 2BASAGLAR (insulin glargine) injection, prescribing information (DailyMed)U.S. Food and Drug Administration / DailyMed (Eli Lilly and Company label) · 2026dailymed.nlm.nih.govAccessed 2 Oct 2026

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